In Annex V to the Biocides Product Regulation the biocidal products are classified into 22 biocidal product-types, grouped in four main areas.
Veterinary Hygiene surface disinfectant products are classed under the PT3 classification.

PT 3 – Veterinary Hygiene
Used for veterinary hygiene purposes such as disinfectants, disinfecting soaps, oral or corporal hygiene products or with anti-microbial function.
Used to disinfect the materials and surfaces associated with the housing or transportation of animals.

The basic requirement for efficacy claims are bacteria and yeasts, however should a disinfectant make a claim against viruses, mycobacteria, spores etc the optional tests must be undertaken to claim suspension or surface test efficacy.


  Veterinary Test ProtocolMax Contact TimePass Criteria
BacteriaBasic requirement – 2,1 test (suspension)EN 165630 min5 log reduction
BacteriaBasic requirement – 2,2 test (surface carrier)EN 14349/1643730 min4 log reduction
YeastBasic requirement- 2,1 test (suspension)EN 165730 min4 log reduction
YeastBasic requirement – 2,2 test (surface carrier)EN 1643830 min4 log reduction
VirusesOptional – 2,1 test (suspension)EN 1467530 min3 log reduction
MycobacteriaOptional – 2,1 test (suspension)EN 1420430 min4 log reduction
FungiOptional – 2,1 test (suspension)EN 165730 min5 log reduction
FungiOptional – 2,2 test (surface carrier)EN 1643830 min4 log reduction
EndoparasitesOptionalDGV30 min3 log reduction


Below is the list of BASIC REQUIREMENT micro-organisms that must have been independently tested under the above veterinary test protocols whilst achieving the minimum log reduction or greater within the maximum contact time to claim efficacy against either bacteria, yeasts, fungi, viruses, mycobacteria, spores and endoparasites. For instance if a disinfectant shows a Log 3.7 reduction against a Canine Parvovirus the product has not passed the required Log 4 or above minimum reduction, thus has NOT passed the test and should not be marketed as effective against such micro-organism.

Required Bacteria organisms
Staphylococcus aureus ATCC 6538
Pseudomonas aeruginosa ATCC 15442
Enterococcus hirae ATCC 10541
Proteus vulgaris ATCC 13315

Required Yeasts organisms
Candida albicans ATCC 10231

Required Fungi organisms
Aspergillus brasiliensis** ATCC 16404

Required Virus organisms
Bovine Entrovirus Type 1, ECBO – Virus ATCC VR-248 (EN14675)
Polio virus type 1, LSc-2ab (Picornavirus) (EN14476)
Adenovirus, type 5, strain Adenoid 75, ATCC VR-5. (EN14476)
Murine norovirus, strain S99 Berlin (EN14476)

Required Mycobacteria organisms
Mycobacterium terrae ATCC 15755
Mycobacterium avium ATCC 15769

Required Spores organisms
Spores of Bacillus subtilis ATCC 6633

Required Endoparasites organisms
Oocysts of Eimeria tenella strain Houghton