In Annex V to the Biocides Product Regulation the biocidal products are classified into 22 biocidal product-types, grouped in four main areas.
Veterinary Hygiene surface disinfectant products are classed under the PT3 classification.

PT 3 – Veterinary Hygiene
Used for veterinary hygiene purposes such as disinfectants, disinfecting soaps, oral or corporal hygiene products or with anti-microbial function.
Used to disinfect the materials and surfaces associated with the housing or transportation of animals.

The basic requirement for efficacy claims are bacteria and yeasts, however should a disinfectant make a claim against viruses, mycobacteria, spores etc the optional tests must be undertaken to claim suspension or surface test efficacy.


Veterinary Test Protocol Max Contact Time Pass Criteria
Bacteria Basic requirement – 2,1 test (suspension) EN 1656 30 min 5 log reduction
Bacteria Basic requirement – 2,2 test (surface carrier) EN 14349/16437 30 min 4 log reduction
Yeast Basic requirement- 2,1 test (suspension) EN 1657 30 min 4 log reduction
Yeast Basic requirement – 2,2 test (surface carrier) EN 16438 30 min 4 log reduction
Viruses Optional – 2,1 test (suspension) EN 14675 30 min 3 log reduction
Mycobacteria Optional – 2,1 test (suspension) EN 14204 30 min 4 log reduction
Fungi Optional – 2,1 test (suspension) EN 1657 30 min 5 log reduction
Fungi Optional – 2,2 test (surface carrier) EN 16438 30 min 4 log reduction
Endoparasites Optional DGV 30 min 3 log reduction


Below is the list of BASIC REQUIREMENT micro-organisms that must have been independently tested under the above veterinary test protocols whilst achieving the minimum log reduction or greater within the maximum contact time to claim efficacy against either bacteria, yeasts, fungi, viruses, mycobacteria, spores and endoparasites. For instance if a disinfectant shows a Log 3.7 reduction against a Canine Parvovirus the product has not passed the required Log 4 or above minimum reduction, thus has NOT passed the test and should not be marketed as effective against such micro-organism.

Required Bacteria organisms
Staphylococcus aureus ATCC 6538
Pseudomonas aeruginosa ATCC 15442
Enterococcus hirae ATCC 10541
Proteus vulgaris ATCC 13315

Required Yeasts organisms
Candida albicans ATCC 10231

Required Fungi organisms
Aspergillus brasiliensis** ATCC 16404

Required Virus organisms
Bovine Entrovirus Type 1, ECBO – Virus ATCC VR-248 (EN14675)
Polio virus type 1, LSc-2ab (Picornavirus) (EN14476)
Adenovirus, type 5, strain Adenoid 75, ATCC VR-5. (EN14476)
Murine norovirus, strain S99 Berlin (EN14476)

Required Mycobacteria organisms
Mycobacterium terrae ATCC 15755
Mycobacterium avium ATCC 15769

Required Spores organisms
Spores of Bacillus subtilis ATCC 6633

Required Endoparasites organisms
Oocysts of Eimeria tenella strain Houghton